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| de los Reyes,Mónica; Palomino,Jaime; Martínez,Víctor; Aretio,Carolina; Gutiérrez,Michel. |
| We evaluated the effect of time and temperature on acrosin release from the acrosomal cap and the activity of this enzyme during in vitro capacitation in fresh and frozen/thawed dog sperm. Sperm-rich fractions of six ejaculates from three dogs were processed as fresh and frozen samples. Each sperm sample was incubated in canine capacitation medium (CCM) for 0, 1, 2 and 3 h at 20°C and at 37°C. After incubation, the samples were assessed by the indirect immunofluorescent staining technique. The probability of having unlabeled sperm (PUS), indicating acrosin loss, was modelled by a binomial distribution using logistic regression. There was a linear relationship between PUS and time at both temperatures (p<0.001); however, a major percentage of unlabeled... |
| Tipo: Journal article |
Palavras-chave: Acrosin; Capacitation; Dog sperm; Cryopreservation. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200005 |
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| Verastegui,Javier; Martínez,Víctor; Roca,Willy; de Peña,Myriam; Gil,Lionel. |
| The Organization of American States is supporting the project "Biosafety Regulations in Latin America and The Caribbean within the framework of the International Biosafety Protocol". The general objective is to strengthen national skills for the assessment and management of risks of biotechnology food products, and to build up public awareness about their benefits and risks in the participant countries, with the ultimate goal to promote their safe and sustainable use within a protective and trusting environment for the public. The first phase has been focused on the evaluation of the political and regulatory systems in Chile, Peru and Colombia, identification of needs, and development of a series of biosafety seminar-workshops in the three countries, with... |
| Tipo: Journal article |
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| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000100007 |
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